Isolation and characterization of the adenylate cyclase structural gene of Neurospora crassa

Jpn J Genet. 1991 Jun;66(3):317-34. doi: 10.1266/jjg.66.317.

Abstract

A single gene (nac) encoding an adenylate cyclase was cloned from the genomic DNA library of Neurospora crassa, using the DNA fragment encoding the catalytic domain of adenylate cyclase of Saccharomyces cerevisiae as a probe. The open reading frame of this gene (6900 base pairs) was interrupted three time by introns. The protein encoded consists of 2300 amino acids and has adenylate cyclase activity. N. crassa adenylate cyclase has a high degree of homology with the catalytic domains of yeast and bovine brain adenylate cyclases.

MeSH terms

  • Adenylyl Cyclases / genetics*
  • Adenylyl Cyclases / isolation & purification
  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA, Fungal
  • Escherichia coli / genetics
  • Genes, Fungal*
  • Genomic Library
  • Molecular Sequence Data
  • Neurospora crassa / enzymology
  • Neurospora crassa / genetics*
  • Neurospora crassa / growth & development
  • Polymorphism, Restriction Fragment Length
  • Restriction Mapping
  • Sequence Alignment

Substances

  • DNA, Fungal
  • Adenylyl Cyclases

Associated data

  • GENBANK/D00909