Isolation of a family of Drosophila POU domain genes expressed in early development

Mech Dev. 1991 Jun;34(2-3):75-84. doi: 10.1016/0925-4773(91)90045-8.

Abstract

The POU domain is a approximately 140 amino acid domain shared by several mammalian transcriptional activators. We have isolated genomic and cDNA clones from three Drosophila POU domain genes, designated pdm-1, pdm-2, and Cf1a. All three genes encode a conserved POU-specific domain and a POU homeo domain but are otherwise divergent. By Northern analysis, all three of the genes are expressed in early embryos; the pdm-1 and Cf1a genes are also expressed at lower levels throughout development. The spatial expression patterns of the pdm-2 and Cf1a genes show that they probably play multiple roles during development: an early role in specific ectodermal cells, and a subsequent role in the embryonic nervous system.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Cloning, Molecular
  • DNA / genetics
  • DNA / isolation & purification*
  • Drosophila / embryology
  • Drosophila / genetics*
  • Gene Expression
  • Genes / genetics*
  • Genes, Homeobox / genetics*
  • Molecular Sequence Data
  • Morphogenesis / genetics
  • Polymerase Chain Reaction
  • Transcription, Genetic / genetics

Substances

  • DNA

Associated data

  • GENBANK/M81957
  • GENBANK/M81958
  • GENBANK/M81959
  • GENBANK/S78944
  • GENBANK/S78945
  • GENBANK/S78946
  • GENBANK/S78947
  • GENBANK/S78948
  • GENBANK/S78949
  • GENBANK/S78950