Establishment of H2O2-induced premature senescence in human fibroblasts concomitant with increased cellular production of H2O2

Ann N Y Acad Sci. 2006 May:1067:210-6. doi: 10.1196/annals.1354.025.


Premature senescence of human fibroblasts is established after exposure to an acute sublethal concentration of H(2)O(2). Overexpression of transforming growth factor-beta1 (TGF-beta1) was shown to be responsible for the appearance of the biomarkers of senescence in these conditions. Other studies have shown that incubation of human fibroblasts with TGF-beta1 leads to overexpression of H(2)O(2). In this work, we show an increased production of H(2)O(2) by human fibroblasts as premature senescence is established after an initial exposure to H(2)O(2).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cells, Cultured
  • Cellular Senescence / drug effects*
  • Fibroblasts / cytology*
  • Fibroblasts / metabolism*
  • Humans
  • Hydrogen Peroxide / analysis
  • Hydrogen Peroxide / metabolism
  • Hydrogen Peroxide / pharmacology*
  • Lung / cytology
  • Lung / embryology
  • Models, Biological
  • Oxidants / analysis
  • Oxidants / metabolism
  • Oxidants / pharmacology*
  • Oxidative Stress
  • Transforming Growth Factor beta / metabolism
  • Transforming Growth Factor beta1


  • Oxidants
  • TGFB1 protein, human
  • Transforming Growth Factor beta
  • Transforming Growth Factor beta1
  • Hydrogen Peroxide