Redox status impairment in liver and kidney of prematurely senescent mice: effectiveness of DTS phytotherapeutic compound

Ann N Y Acad Sci. 2006 May;1067:408-13. doi: 10.1196/annals.1354.058.

Abstract

T-maze test-selected prematurely senescent mice (PSM) were allocated into two groups: (A) those given DTS (150 mg/kg) orally for 30 days and (B) untreated PSM with age-matched fast T-maze performers as control. After sacrifice, the liver and kidney were analyzed for catalase (CAT) activity, glutathione peroxidase (GPx), superoxide dismutase (SOD), malondyaldehyde (MDA), and plasma thiols. Untreated PSM showed decreased plasma thiols and tissue level of CAT, SOD, GPx, with higher MDA (P < 0.01 vs. fast performers), while DTS (Denshichi-Tochiu-Sen) significantly improved glutathione and cysteine (P < 0.05) and tissue concentration of the above parameters (P < 0.05). Such preliminary data suggest that DTS mitigated oxidative damage in PSM, with likely action on the cytoplasm and mitochondrial matrix.

Publication types

  • Comparative Study

MeSH terms

  • Aging / metabolism*
  • Animals
  • Catalase / blood
  • Glutathione Peroxidase / blood
  • Glutathione Reductase / blood
  • Kidney / blood supply
  • Kidney / drug effects*
  • Kidney / enzymology
  • Kidney / metabolism
  • Liver / blood supply
  • Liver / drug effects*
  • Liver / enzymology
  • Liver / metabolism
  • Malondialdehyde / analysis
  • Mice
  • Mice, Inbred BALB C
  • Oxidation-Reduction
  • Oxidative Stress
  • Phytotherapy
  • Random Allocation
  • Sulfonamides / pharmacology*
  • Superoxide Dismutase / blood
  • Thiadiazoles / pharmacology*
  • Thiobarbituric Acid Reactive Substances / analysis

Substances

  • 2-(2,2-dichloroacetylamino)-1,3,4-thiadiazole-5-sulfonamide
  • Sulfonamides
  • Thiadiazoles
  • Thiobarbituric Acid Reactive Substances
  • Malondialdehyde
  • Catalase
  • Glutathione Peroxidase
  • Superoxide Dismutase
  • Glutathione Reductase