Frequent allelic losses and mutations of the p53 gene in human ovarian cancer

Cancer Res. 1991 Oct 1;51(19):5171-6.


The p53 gene on chromosome 17p is considered to be a tumor suppressor gene, and frequent mutations of the p53 gene have been found in a wide variety of human cancers. We examined 31 ovarian cancers for allelic losses and mutations of the p53 gene by polymerase chain reaction-single strand conformation polymorphism analysis as well as restriction fragment length polymorphism analysis. Allelic loss of the p53 gene was detected in 16 of 20 cases (80%). Mutations were detected in 9 of 31 cases (29%): 2 cases in exon 4; 5 cases in exons 5-6; and 2 cases in exons 7-8. In 8 of 9 cases, p53 mutations were accompanied by losses of the normal allele. These alterations of the p53 gene were commonly detected from stage I to stage IV. These results suggest that alterations of the p53 gene play an important role in the development of human ovarian cancers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Chromosomes, Human, Pair 17*
  • Cloning, Molecular
  • Electrophoresis, Agar Gel
  • Exons
  • Female
  • Genes, p53*
  • Humans
  • Molecular Sequence Data
  • Mutation*
  • Ovarian Neoplasms / genetics*
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length

Associated data

  • GENBANK/M60710
  • GENBANK/M60711
  • GENBANK/M60712
  • GENBANK/M60713
  • GENBANK/M60714
  • GENBANK/M60715
  • GENBANK/M60716
  • GENBANK/S62213
  • GENBANK/S62216
  • GENBANK/S73009