Novel comparison of capillary electrophoresis versus immunoassay in the measurement of total percent carbohydrate deficient transferrin

Clin Biochem. 2006 Aug;39(8):826-32. doi: 10.1016/j.clinbiochem.2006.04.022. Epub 2006 May 19.

Abstract

Objectives: Novel comparison of CDT isoforms as determined by CE with an FDA-approved immunoassay kit.

Design and methods: Subjects (n=51) were categorized by drinking status based on AUDIT questionnaire responses. CDT isoform analyses by CE were compared to a commercially available, FDA-approved immunoassay. The analytical specificity of the immunoassay kit was assessed by analysis with IEF.

Results: Because of the poor correlation between % CDT as measured by CE and the TIA immunoassay and between subject-reported drinking levels and results from the TIA assay, extraction column eluants from the immunoassays were analyzed by IEF for analytical specificity. % CDT by TIA included some trisialo-Tf, a non-CDT fraction, in the % CDT determination.

Conclusions: Total % CDT by CE, which separates all isoforms is more analytically specific than immunoassays because it does not include trisialo-Tf in the CDT calculation.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adolescent
  • Adult
  • Electrophoresis, Capillary / methods*
  • Female
  • Humans
  • Immunoassay / methods*
  • Isoelectric Focusing
  • Male
  • Middle Aged
  • Transferrin / analogs & derivatives*
  • Transferrin / analysis

Substances

  • Transferrin
  • carbohydrate-deficient transferrin