Ingestion of IH636 grape seed proanthocyanidin extract to prevent selenite-induced oxidative stress in experimental cataract

J Cataract Refract Surg. 2006 Jun;32(6):1041-5. doi: 10.1016/j.jcrs.2006.02.041.


Purpose: To investigate whether dietary supplementation with IH636 grape seed proanthocyanidin extract (GSPE) prevents selenite-induced cataract.

Setting: Department of Ophthalmology, Gulhane Military Medical Academy, Ankara, Turkey.

Methods: Thirty Spraque-Dawley rat litters were put randomly into 3 groups. In group 1 (n = 10), sodium selenite (30 nmol/g body weight) was injected subcutaneously on postpartum day 10. In group 2 (n = 10), sodium selenite (30 nmol/g body weight) was injected on postpartum day 10 and oral GSPE (100 mg/kg body weight) was given for 1 week after sodium selenite injection. Only subcutaneous saline was injected in group 3 (control, n = 10). The development of cataract was assessed for 3 weeks, and its density was graded and photographed with a slitlamp. Removed rat lenses were analyzed for glutathione (GSH) and malondialdehyde (MDA).

Results: All of the rats in group 1 had cataract between stage 6 and stage 3. In group 2, only 5 of 10 eyes had cataract between stage 3 and stage 2 and no cataract occurred in the remaining 5 rats. The difference between mean cataract stages in group 1 and group 2 was significant (P<.05). The mean GSH level in group 1 was significantly lower than in group 2 and controls (P<.05). The mean MDA level in group 1 was significantly higher than in group 2 and controls (P<.05).

Conclusions: IH636 grape seed proanthocyanidin extract effectively suppressed cataract formation in rats. Routine consumption of grape seed proanthocyanidin extract in the form of food or dietary supplement may offer a prophylactic measure against onset and progression of cataract.

MeSH terms

  • Administration, Oral
  • Animals
  • Cataract / chemically induced
  • Cataract / metabolism
  • Cataract / pathology
  • Cataract / prevention & control*
  • Diet
  • Disease Models, Animal
  • Female
  • Free Radical Scavengers / administration & dosage*
  • Glutathione / metabolism
  • Grape Seed Extract
  • Lens, Crystalline / drug effects*
  • Lens, Crystalline / metabolism
  • Lens, Crystalline / pathology
  • Male
  • Malondialdehyde / metabolism
  • Oxidative Stress / drug effects*
  • Plant Extracts / administration & dosage*
  • Proanthocyanidins / administration & dosage*
  • Rats
  • Rats, Sprague-Dawley
  • Sodium Selenite / toxicity*


  • Free Radical Scavengers
  • Grape Seed Extract
  • Grape Seed Proanthocyanidins
  • Plant Extracts
  • Proanthocyanidins
  • Malondialdehyde
  • Glutathione
  • Sodium Selenite