The course of allergen-induced leukocyte infiltration in human and experimental asthma

J Allergy Clin Immunol. 2006 Jul;118(1):91-7. doi: 10.1016/j.jaci.2006.02.034. Epub 2006 Apr 27.


Background: Although the timing of allergen-induced bronchoconstriction is well defined, there is little information about the kinetics of allergen-induced leukocyte infiltration in asthma and its comparability between human and animal models of asthma.

Objective: To investigate systematically allergen-induced leukocyte infiltration into the airway lumen in human and experimental asthma by using bronchoalveolar lavage.

Methods: Patients with allergic asthma were lavaged at different time points as long as 1 week after segmental allergen challenge. Allergen-sensitized mice were lavaged as long as 3 weeks after allergen challenge. Differential cell counts, lymphocyte subsets, and cytokines were assessed in bronchoalveolar lavage fluid.

Results: In both models, neutrophil infiltration was a relatively early event (maximum: 18 hours after challenge). In contrast, eosinophil infiltration peaked 42 hours (human model) to 4 days (mouse model) after allergen challenge, paralleled by an IL-5 peak in this period. There were elevated macrophage counts over a period of several days after allergen challenge in both models. Lymphocytes (predominantly CD4+ T cells) peaked 18 hours after challenge in the human model, but not until 2 weeks after challenge in the murine model.

Conclusion: Early neutrophil accumulation (within hours after challenge) and delayed eosinophil accumulation (within days after challenge) in the airway lumen are common features of allergen-induced airway inflammation, whereas lymphocyte kinetics are dependent on the asthma model.

Clinical implications: Similarities in the infiltration kinetics of granulocytes after allergen challenge suggest a common role for these cells in asthma, whereas the presumed orchestration of allergic inflammation by lymphocytes appears to differ between the models.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Allergens / immunology*
  • Animals
  • Asthma / immunology*
  • Bronchoalveolar Lavage Fluid / cytology
  • Cell Count
  • Disease Models, Animal
  • Eosinophils / immunology*
  • Female
  • Humans
  • Interferon-gamma / analysis
  • Interleukin-4 / analysis
  • Interleukin-5 / analysis
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Neutrophil Infiltration*


  • Allergens
  • Interleukin-5
  • Interleukin-4
  • Interferon-gamma