Amplification of CCND1, EMS1, PIK3CA, and ERBB oncogenes in ethmoid sinus adenocarcinomas

Otolaryngol Head Neck Surg. 2006 Jul;135(1):135-9. doi: 10.1016/j.otohns.2006.01.014.


Objective: Assess the amplification of CCND1, EMS1, PIK3CA, ERBB1, and ERBB2 oncogenes in ethmoid sinus adenocarcinomas.

Study design and setting: Tissue samples from 13 primary ethmoid adenocarcinomas and 2 recurrences were studied at Hospital Central de Asturias, Oviedo, Spain, between July 1998 and February 2002. A semiquantitative evaluation of CCND1, EMS1, PIK3CA, ERBB1, and ERBB2 amplification was performed by multiplex polymerase chain reaction.

Results: Three (23%) cases presented CCND1 amplification, one of them with a concurrent PIK3CA amplification. EMS1 was amplified in the 2 studied recurrences. ERBB1 was amplified in 1 case (8%), whereas none presented ERBB2 amplification. Oncogenic amplifications were only detected in advanced (stage III) tumors; however, no prognostic value could be shown for them.

Conclusions: CCND1, EMS1, PIK3CA, and ERBB1 amplifications are uncommon and appear to be late events in the development of ethmoid sinus adenocarcinoma.

Significance: New information on the carcinogenesis of this infrequent sinonasal tumor is presented.

Publication types

  • Comparative Study

MeSH terms

  • Adenocarcinoma / genetics*
  • Adenocarcinoma / metabolism
  • Adenocarcinoma / pathology
  • Aged
  • Biomarkers, Tumor / genetics
  • Class I Phosphatidylinositol 3-Kinases
  • Cortactin / genetics*
  • Cyclin D1 / genetics*
  • DNA, Neoplasm / genetics
  • Ethmoid Sinus*
  • Gene Expression Regulation, Neoplastic
  • Genes, erbB-1 / genetics*
  • Humans
  • In Vitro Techniques
  • Male
  • Middle Aged
  • Paranasal Sinus Neoplasms / genetics*
  • Paranasal Sinus Neoplasms / metabolism
  • Paranasal Sinus Neoplasms / pathology
  • Phosphatidylinositol 3-Kinases / genetics*
  • Polymerase Chain Reaction


  • Biomarkers, Tumor
  • CTTN protein, human
  • Cortactin
  • DNA, Neoplasm
  • Cyclin D1
  • Phosphatidylinositol 3-Kinases
  • Class I Phosphatidylinositol 3-Kinases
  • PIK3CA protein, human