The purpose of this study was to compare knee kinematics in patients with bi-cruciate preserving total knee arthroplasty and posterior cruciate ligament (PCL) preserving total knee arthroplasty. Five knees received PCL-retaining arthroplasty and nine knees received both cruciate-retaining arthroplasty (ACL/PCL knees). We studied treadmill gait, stair stepping, and maximum flexion activities using lateral fluoroscopy and shape matching. For maximum flexion, the ACL/PCL knees showed 6 mm more posterior translation of the lateral condyle (p < 0.05). For the stair activity, posterior translations of the lateral condyle were significantly greater in the ACL/PCL knees from 30 degrees to 70 degrees flexion (p < 0.05). Both condyles in the ACL/PCL knees showed greater posterior translation in the stance and swing phases of gait than in the PCL knees (p < 0.05). Preserving both cruciate ligaments in total knee arthroplasty appears to maintain some basic features of normal knee kinematics in these activities.