Foxp1 is an essential transcriptional regulator of B cell development

Nat Immunol. 2006 Aug;7(8):819-26. doi: 10.1038/ni1358. Epub 2006 Jul 2.


Forkhead transcription factors are key participants in development and immune regulation. Here we demonstrate that absence of the gene encoding the forkhead transcription factor Foxp1 resulted in a profound defect in early B cell development. Foxp1 deficiency was associated with decreased expression of all B lineage genes in B220+ fetal liver cells as well as with a block in the transition from pro-B cell to pre-B cell involving diminished expression of recombination-activating genes 1 and 2. Foxp1 bound to the Erag enhancer and was involved in controlling variable-(diversity)-joining recombination of the gene encoding immunoglobulin heavy chain in a B cell lineage-specific way. Our results identify Foxp1 as an essential participant in the transcriptional regulatory network of B lymphopoiesis.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • B-Lymphocytes / cytology*
  • B-Lymphocytes / immunology
  • Base Sequence
  • Blotting, Southern
  • Cell Differentiation / immunology
  • Cell Lineage / immunology*
  • Chimera
  • Electrophoretic Mobility Shift Assay
  • Flow Cytometry
  • Forkhead Transcription Factors / immunology*
  • Gene Expression
  • Genes, RAG-1 / immunology
  • Humans
  • Lymphopoiesis / physiology*
  • Mice
  • Mice, Knockout
  • Molecular Sequence Data
  • Repressor Proteins / immunology*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Homology, Nucleic Acid
  • Transcription, Genetic / immunology*


  • Forkhead Transcription Factors
  • Foxp1 protein, mouse
  • Repressor Proteins