Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 128 (27), 8738-9

A Genetically Encoded Fluorescent Amino Acid

Affiliations

A Genetically Encoded Fluorescent Amino Acid

Jiangyun Wang et al. J Am Chem Soc.

Abstract

The fluorescent amino acid l-(7-hydroxycoumarin-4-yl) ethylglycine 1 has been genetically encoded in E. coli in response to the amber TAG codon. Because of its high fluorescence quantum yield, relatively large Stoke's shift, and sensitivity to both pH and polarity, this amino acid should provide a useful probe of protein localization and trafficking, protein conformation changes, and protein-protein interactions.

Similar articles

  • An Enhanced System for Unnatural Amino Acid Mutagenesis in E. Coli
    TS Young et al. J Mol Biol 395 (2), 361-74. PMID 19852970.
    We report a new vector, pEVOL, for the incorporation of unnatural amino acids into proteins in Escherichia coli using evolved Methanocaldococcus jannaschii aminoacyl-tRNA …
  • An Expanded Genetic Code With a Functional Quadruplet Codon
    JC Anderson et al. Proc Natl Acad Sci U S A 101 (20), 7566-71. PMID 15138302.
    With few exceptions the genetic codes of all known organisms encode the same 20 amino acids, yet all that is required to add a new building block are a unique tRNA/aminoa …
  • Genetic Introduction of a Diketone-Containing Amino Acid Into Proteins
    H Zeng et al. Bioorg Med Chem Lett 16 (20), 5356-9. PMID 16934461.
    An orthogonal tRNA/aminoacyl-tRNA synthetase pair was evolved that makes possible the site-specific incorporation of an unnatural amino acid bearing a beta-diketone side …
  • A Chemical Toolkit for Proteins--An Expanded Genetic Code
    J Xie et al. Nat Rev Mol Cell Biol 7 (10), 775-82. PMID 16926858. - Review
    Recently, a method to encode unnatural amino acids with diverse physicochemical and biological properties genetically in bacteria, yeast and mammalian cells was developed …
  • Adding Amino Acids to the Genetic Repertoire
    J Xie et al. Curr Opin Chem Biol 9 (6), 548-54. PMID 16260173. - Review
    Considerable progress has been made in expanding the number and nature of genetically encoded amino acids in Escherichia coli, yeast and mammalian cells in the past four …
See all similar articles

Cited by 74 PubMed Central articles

See all "Cited by" articles

Publication types

MeSH terms

LinkOut - more resources

Feedback