The route of lysine breakdown in Candida tropicalis

FEMS Microbiol Lett. 1991 Aug 1;66(2):209-13. doi: 10.1016/0378-1097(91)90334-7.

Abstract

Candida tropicalis was found to contain high levels of the following enzymes after growth in defined medium on L-lysine as sole nitrogen source: L-lysine N6-acetyltransferase, N6-acetyl-lysine aminotransferase, and aminotransferase activity for 5-aminovalerate and 4-aminobutyrate. Extracts were also capable of converting 5-acetamidovalerate (and 4-acetamidobutyrate) to acetate. N6-Acetyllysine however, only gave rise to acetate in the presence of 2-oxoglutarate, NAD+ and thiamine pyrophosphate. These activities were undetectable or present in much lower concentrations in cells that had been grown on ammonium sulphate as sole nitrogen source. It is concluded that L-lysine is degraded in this organism via N6-acetyllysine, 5-acetamidovalerate and 5-aminovalerate, both nitrogen atoms being removed by transamination.

MeSH terms

  • Acetates / analysis
  • Acetic Acid
  • Acetyltransferases / metabolism
  • Candida / enzymology
  • Candida / metabolism*
  • Glutamates / analysis
  • Glutamic Acid
  • Kinetics
  • Lysine / analogs & derivatives*
  • Lysine / metabolism*
  • Rhodotorula / enzymology
  • Rhodotorula / metabolism
  • Temperature
  • Transaminases / metabolism

Substances

  • Acetates
  • Glutamates
  • Glutamic Acid
  • N-epsilon-acetyllysine
  • Acetyltransferases
  • Transaminases
  • Lysine
  • Acetic Acid