Mapping protein-protein interactions by mass spectrometry

Curr Opin Biotechnol. 2006 Aug;17(4):394-9. doi: 10.1016/j.copbio.2006.06.008. Epub 2006 Jul 5.


Mass spectrometry is currently at the forefront of technologies for mapping protein-protein interactions, as it is a highly sensitive technique that enables the rapid identification of proteins from a variety of biological samples. When used in combination with affinity purification and/or chemical cross-linking, whole or targeted protein interaction networks can be elucidated. Several methods have recently been introduced that display increased specificity and a reduced occurrence of false-positives. In the future, information gained from human protein interaction studies could lead to the discovery of novel pathway associations and therapeutic targets.

Publication types

  • Review

MeSH terms

  • Animals
  • Chromatography, Affinity
  • Cross-Linking Reagents / chemistry
  • Humans
  • Mass Spectrometry / methods*
  • Protein Binding / physiology
  • Protein Interaction Mapping / methods*
  • Proteome / genetics
  • Proteome / metabolism
  • Reproducibility of Results
  • Signal Transduction / physiology


  • Cross-Linking Reagents
  • Proteome