Some monoclonal antibody reagents (C219 and JSB-1) to P-glycoprotein contain antibodies to blood group A carbohydrate determinants: a problem of quality control for immunohistochemical analysis

J Histochem Cytochem. 1991 Dec;39(12):1603-10. doi: 10.1177/39.12.1682363.

Abstract

Monoclonal antibodies (MAb) C219 and JSB-1 have been used extensively in the analysis of P-glycoprotein expression in normal and malignant tissues. This study demonstrates that some commercial lots of these MAb, even those supplied as purified immunoglobulins, contain contaminating anti-A blood group antibodies. In both sources of reagent, the antibody was specific for a particular A structure, known as repetitive or Type 3 A. These observations may account for earlier studies showing polymorphic variation in P-glycoprotein expression in epithelial tissues and an apparent correlation with the A blood type of the donor. Such reactivity can be eliminated by absorption of anti-P-glycoprotein reagents with A erythrocytes. These data re-emphasize the importance of evaluating MAb samples for unsuspected contaminating antibodies.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • ABO Blood-Group System / immunology*
  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • Animals
  • Antibodies, Monoclonal / immunology*
  • Carbohydrate Sequence
  • Carbohydrates / immunology*
  • Cell Line
  • Chromatography, Thin Layer
  • Erythrocytes / metabolism
  • Fallopian Tubes / metabolism
  • Female
  • Glycolipids / immunology
  • Hemagglutination Tests
  • Humans
  • Immunohistochemistry
  • Isoantibodies / immunology*
  • Isoantigens / immunology
  • Membrane Glycoproteins / immunology*
  • Molecular Sequence Data
  • Ovarian Neoplasms / metabolism
  • Ovary / metabolism
  • Quality Control
  • Swine

Substances

  • ABO Blood-Group System
  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • Antibodies, Monoclonal
  • Carbohydrates
  • Glycolipids
  • Isoantibodies
  • Isoantigens
  • Membrane Glycoproteins