Down-regulation of Jagged-1 induces cell growth inhibition and S phase arrest in prostate cancer cells

Int J Cancer. 2006 Nov 1;119(9):2071-7. doi: 10.1002/ijc.22077.


Notch is an ancient cell signaling system that regulates cell fate specification, stem cell maintenance and initiation of differentiation in many tissues. It has been reported that Jagged-1, a Notch ligand, is significantly over expressed in metastatic prostate cancer compared to localized prostate cancer or benign prostatic tissues. Therefore, deregulation of Jagged-1 protein levels may play a role in prostate cancer cell growth and progression. Hence, the aim of our current study was to investigate the mechanistic role of Jagged-1 in prostate cancer cell growth and cell cycle progression. Our results show, for the first time, that down-regulation of Jagged-1 induces cell growth inhibition and S phase cell cycle arrest in prostate cancer cells, with reduced CDK2 kinase activity and increased p27 expression. These results suggest that Jagged-1 could be a potential therapeutic target for the treatment of prostate cancer.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Retracted Publication

MeSH terms

  • Calcium-Binding Proteins / genetics*
  • Cell Division
  • Cell Line, Tumor
  • Cyclin-Dependent Kinase 2 / metabolism
  • Disease Progression
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Intercellular Signaling Peptides and Proteins / genetics*
  • Jagged-1 Protein
  • Male
  • Membrane Proteins / genetics*
  • Prostatic Neoplasms / enzymology
  • Prostatic Neoplasms / pathology*
  • RNA, Small Interfering / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Serrate-Jagged Proteins


  • Calcium-Binding Proteins
  • Intercellular Signaling Peptides and Proteins
  • JAG1 protein, human
  • Jagged-1 Protein
  • Membrane Proteins
  • RNA, Small Interfering
  • Serrate-Jagged Proteins
  • Cyclin-Dependent Kinase 2