Understanding and making use of human memory B cells

Immunol Rev. 2006 Jun;211(1):303-9. doi: 10.1111/j.0105-2896.2006.00403.x.


The work of our laboratory has focused on the study of human memory B cells. Using an in vitro approach we dissected the triggering requirements of B cells and unveiled a distinct role for TLRs in the activation of naive versus memory B cells. Using an ex vivo approach we analyzed the dynamics of memory B cells and ASCs and the kinetics of serum antibodies during secondary immune responses and in steady state conditions and used these quantitative data to build up a model of serological memory. According to this model memory B cells behave as ;stem cells' capable of generating plasma cells and antibodies in an antigen-dependent as well as in an antigen-independent fashion. Finally we developed an efficient method to interrogate human memory B cells and to isolate human monoclonal antibodies. This method can be exploited for the production of neutralizing antibodies for serotherapy and for "analytic vaccinology".

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Antibody-Producing Cells / immunology
  • B-Lymphocytes / immunology*
  • Humans
  • Immunization, Secondary
  • Immunologic Memory / immunology*
  • Lymphocyte Activation / immunology
  • Mice
  • Toll-Like Receptors / immunology
  • Vaccination


  • Antibodies, Monoclonal
  • Toll-Like Receptors