Lactobacillus tucceti sp. nov., a new lactic acid bacterium isolated from sausage

Syst Appl Microbiol. 2006 Jul;29(5):389-95. doi: 10.1016/j.syapm.2005.09.006. Epub 2005 Oct 19.

Abstract

Following the application of several molecular techniques strain R 19c, isolated from sausage by Reuter in 1970 and deposited at the DSMZ as Lactobacillus sp., has been identified as pertaining to a new species. It showed singular ISR-DdeI and ISR-HaeIII profiles that allowed its differentiation from 68 lactic acid bacteria reference strains analyzed. Phylogenetic analysis based on 16S rRNA gene sequences places this strain in the genus Lactobacillus within the Lactobacillus alimentarius group. Species L. versmoldensis is the closest phylogenetic neighbor with 96.3% sequence similarity. DNA-DNA hybridization experiments confirmed the independent status at species level of this strain. Species-specific primers for PCR detection of this new species have been developed. Phenotypically it can be distinguished from the closest relative L. versmoldensis by several traits such as the peptidoglycan type (L-Lys-Gly-D-Asp), acid production from L-rhamnose, D-mannitol and L-fucose and its inability to ferment d-galactose, d-melibiose and d-sucrose. The name Lactobacillus tucceti sp. nov. is proposed with strain R 19c(T) (=DSM 20183(T)= CECT 5920(T)) as the type strain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA, Ribosomal / analysis
  • Deoxyribonucleases, Type II Site-Specific / metabolism
  • Lactobacillus / classification
  • Lactobacillus / isolation & purification*
  • Lactobacillus / metabolism
  • Meat Products / microbiology*
  • Nucleic Acid Hybridization
  • Phenotype
  • Phylogeny
  • Polymerase Chain Reaction

Substances

  • DNA, Ribosomal
  • endodeoxyribonuclease DdeI
  • Deoxyribonucleases, Type II Site-Specific
  • GGCC-specific type II deoxyribonucleases