Measurement of unsaturated ""granulocyte-related'' (TC I and TC III) and ""liver-related'' (TC II) B12 binders by instant batch separation using a microfine precipitate of silica (QUSO G32)

J Lab Clin Med. 1975 Sep;86(3):505-12.


A microfine precipitate of silica (Quso G32) almost instantly quantitatively adsorbs the polypeptide transcobalamin II from serum without adsorbing the glycoproteins transcobalamins I and III. This provides a simple, rapid, and reproducible method for the measurement of the granulocyte-related transcobalamins I and III and the liver-related transcobalamin II in serum, and is also applicable to their purification, and the separation and purification of other polypeptide-glycoprotein mixtures.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Blood Proteins / analysis*
  • Chromatography, Gel
  • Glycoproteins / analysis
  • Granulocytes
  • Liver
  • Peptides / analysis
  • Radioligand Assay
  • Silicon Dioxide
  • Transcobalamins / analysis*


  • Blood Proteins
  • Glycoproteins
  • Peptides
  • Transcobalamins
  • Silicon Dioxide