FREALIGN: high-resolution refinement of single particle structures

J Struct Biol. 2007 Jan;157(1):117-25. doi: 10.1016/j.jsb.2006.05.004. Epub 2006 Jun 2.


The refinement of three-dimensional reconstructions and correction for the contrast transfer function of the microscope are important steps in the determination of macromolecular structures by single particle electron microscopy. The algorithms implemented in the computer program FREALIGN are optimized to perform these tasks efficiently. A general overview and details on how to use FREALIGN are provided. The program is free and available for download on the author's web page.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms
  • Image Enhancement / methods*
  • Imaging, Three-Dimensional / methods*
  • Microscopy, Electron
  • Particle Size
  • Software Design
  • Software*