Storage and oxidation of long-chain fatty acids in the C57/BL6 mouse heart as measured by NMR spectroscopy

FEBS Lett. 2006 Jul 24;580(17):4282-7. doi: 10.1016/j.febslet.2006.06.068. Epub 2006 Jun 30.


Triglyceride turnover in the isolated C57/BL6 mouse heart was measured by dynamic 13C edit-(1)H observe NMR and the rate of fatty acid oxidation was determined by 13C NMR isotopomer analysis. In the presence of a physiological mixture of substrates, energy was produced in the citric acid cycle by oxidation of long-chain fatty acids (18%), ketones (34%), lactate (24%), pyruvate (7%), and other sources (17%). Exogenous fatty acids appeared in the triglyceride pool at 0.24 micromol/g dry wt/min, similar to the rate of oxidation of long-chain fatty acids, 0.16 micromol/g dry wt/min. Isoproterenol decreased the rate of de novo triglyceride synthesis and increased the rate of fatty acid oxidation.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Cardiotonic Agents / pharmacology
  • Citric Acid Cycle / drug effects
  • Citric Acid Cycle / physiology
  • Fatty Acids / metabolism*
  • Heart / physiology*
  • Isoproterenol / pharmacology
  • Lactic Acid / metabolism
  • Lipid Metabolism / drug effects
  • Lipid Metabolism / physiology*
  • Magnetic Resonance Spectroscopy
  • Mice
  • Organ Culture Techniques
  • Oxidation-Reduction / drug effects
  • Perfusion
  • Pyruvic Acid / metabolism
  • Triglycerides / biosynthesis


  • Cardiotonic Agents
  • Fatty Acids
  • Triglycerides
  • Lactic Acid
  • Pyruvic Acid
  • Isoproterenol