The chemical composition of propolis from two regions in Canada was studied: Boreal forest and Pacific coastal forest that lay outside the area of distribution of Aigeiros poplars, the usual propolis source plants. In the sample from Victoria, p-hydroxyacetophenone, benzyl hydroxybenzoate and cinnamic acid were the major components, accompanied by significant amounts of dihydrochalcones, which allowed the identification of its plant source: Populus trichocarpa of Section Tacamahaca. Three dihydrochalcones were new for propolis. The sample from Richmond was characterized by large amounts of p-coumaric and cinnamic acid, typical for poplars of Section Leuce, subsection Trepidae, its plant source was identified as P. tremuloides. Both samples showed good radical scavenging activity against DPPH. Obviously, Northern type propolis is a promising potential source of biologically active substances and deserves further investigation.