Most of the interleukin 1 receptor antagonist, cathepsin S, macrophage migration inhibitory factor, nerve growth factor, and interleukin 18 release by explants of human adipose tissue is by the non-fat cells, not by the adipocytes

Metabolism. 2006 Aug;55(8):1113-21. doi: 10.1016/j.metabol.2006.04.008.

Abstract

The present studies were designed to compare the relative release of interleukin 1 receptor antagonist (IL-1Ra), cathepsin S, macrophage migration inhibitory factor (MIF), nerve growth factor (NGF), and interleukin 18 (IL-18) by adipocytes as compared with the non-fat cells present in subcutaneous and omental adipose tissue from morbidly obese gastric bypass patients as compared with obese abdominoplasty patients. The release of IL-1Ra, cathepsin S, and MIF by explants of human adipose tissue incubated for 48 hours averaged 6, 9, and 19 pmol/g, respectively, and was far greater than the release of NGF (0.05 pmol/g) or IL-18 (0.006 pmol/g). The release by human adipocytes of IL-1Ra, cathepsin S, and MIF was 0.13, 0.32, and 2.6 pmol/g, respectively, over 48 hours, whereas NGF release was 0.003 and IL-18 0.001 pmol/g. Only the total release of MIF by human adipose tissue explants was enhanced, whereas that of IL-18 was significantly reduced in explants from morbidly obese women. Most of (55%-73%) the release of IL-1Ra, cathepsin S, MIF, NGF, and IL-18 was by the adipose tissue matrix, whereas release by stromal-vascular (SV) cells was 3% to 28% of total release over 48 hours by the adipose tissue matrix, SV cells and adipocytes. The release of NGF by adipocytes was 42%, that of MIF was 27%, and for the other factors 15% or less of release over 48 hours by the adipose tissue matrix, SV cells, and adipocytes. Our results suggest that the non-fat cells in human adipose tissue contribute to most of the release of NGF, IL-18, IL-1Ra, cathepsin S, and MIF seen during primary culture of adipose tissue explants from obese women.

MeSH terms

  • Abdominal Fat / metabolism
  • Adipocytes / metabolism*
  • Adipose Tissue / cytology
  • Adipose Tissue / metabolism*
  • Adult
  • Blood Vessels / cytology
  • Blood Vessels / metabolism
  • Body Composition / physiology
  • Cathepsins / metabolism*
  • DNA / biosynthesis
  • Female
  • Humans
  • Interleukin 1 Receptor Antagonist Protein
  • Interleukin-18 / metabolism*
  • Lipid Metabolism
  • Macrophage Migration-Inhibitory Factors / metabolism*
  • Middle Aged
  • Nerve Growth Factor / metabolism*
  • Organ Culture Techniques
  • RNA / biosynthesis
  • Sialoglycoproteins / metabolism*
  • Stromal Cells / metabolism

Substances

  • IL1RN protein, human
  • Interleukin 1 Receptor Antagonist Protein
  • Interleukin-18
  • Macrophage Migration-Inhibitory Factors
  • Sialoglycoproteins
  • RNA
  • DNA
  • Nerve Growth Factor
  • Cathepsins
  • cathepsin S