Presence of potentially pathogenic Babesia sp. for human in Ixodes ricinus in Switzerland

Ann Agric Environ Med. 2006;13(1):65-70.

Abstract

We have designed and performed a new PCR method based on the 18S rRNA in order to individuate the presence and the identity of Babesia parasites. Out of 1159 Ixodes ricinus (Acari: Ixodidae) ticks collected in four areas of Switzerland, nine were found to contain Babesia DNA. Sequencing of the short amplicon obtained (411-452 bp) allowed the identification of three human pathogenic species: Babesia microti, B. divergens, for the first time in Switzerland, Babesia sp. EU1. We also report coinfections with B. sp. EU1-Borrelia burgdorferi sensu stricto and Babesia sp. EU1-B. afzelii.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arachnid Vectors / parasitology*
  • Babesia / classification
  • Babesia / isolation & purification*
  • Babesia / pathogenicity
  • Babesia microti / isolation & purification
  • Babesia microti / pathogenicity
  • Babesiosis / transmission
  • Base Sequence
  • DNA, Protozoan / chemistry
  • Ixodes / parasitology*
  • Molecular Sequence Data
  • Phylogeny
  • Polymerase Chain Reaction / methods*
  • RNA, Ribosomal, 18S / chemistry
  • Species Specificity
  • Switzerland / epidemiology

Substances

  • DNA, Protozoan
  • RNA, Ribosomal, 18S