Homology of a candidate spermatogenic gene from the mouse Y chromosome to the ubiquitin-activating enzyme E1

Nature. 1991 Dec 12;354(6353):483-6. doi: 10.1038/354483a0.


The Sxr (sex-reversed) region, a fragment of the Y chromosome short arm, can cause chromosomally female XXSxr or XSxrO mice to develop as sterile males. The original Sxr region, termed Sxra, encodes: Tdy, the primary sex-determining gene; Hya, the controlling or structural locus for the minor transplantation antigen H-Y; gene(s) controlling the expression of the serologically detected male antigen (SDMA); Spy, a gene(s) required for the survival and proliferation of A spermatogonia during spermatogenesis; Zfy-1/Zfy-2, zinc-finger-containing genes of unknown function; and Sry, which is probably identical to Tdy. A deletion variant of Sxra, termed Sxrb, which lacks Hya, SDMA expression, Spy and some Zfy-2 sequences, makes positional cloning of these genes possible. We report here the isolation of a new testis-specific gene, Sby, mapping to the DNA deleted from the Sxrb region (the delta Sxrb interval). Sby has extensive homology to the X-linked human ubiquitin-activating enzyme E1. The critical role of this enzyme in nuclear DNA replication together with the testis-specific expression of Sby suggests Sby as a candidate for the spermatogenic gene Spy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Southern
  • Chromosome Mapping
  • Female
  • Gene Expression / genetics
  • Humans
  • Ligases / genetics*
  • Male
  • Mice
  • Molecular Sequence Data
  • Polymorphism, Restriction Fragment Length
  • Sequence Homology, Nucleic Acid*
  • Spermatogenesis / genetics*
  • Ubiquitin-Activating Enzymes
  • Ubiquitin-Protein Ligases
  • X Chromosome
  • Y Chromosome*


  • Ubiquitin-Protein Ligases
  • Ligases
  • Ubiquitin-Activating Enzymes

Associated data

  • GENBANK/M88481
  • GENBANK/S70253
  • GENBANK/S70255
  • GENBANK/S70257
  • GENBANK/S70258
  • GENBANK/S70261
  • GENBANK/S70262
  • GENBANK/X57453
  • GENBANK/X57454
  • GENBANK/X57455
  • GENBANK/X62580
  • GENBANK/X62581