Quantitative profiling of polar glycerolipid species from organs of wild-type Arabidopsis and a phospholipase Dalpha1 knockout mutant

Phytochemistry. 2006 Sep;67(17):1907-24. doi: 10.1016/j.phytochem.2006.06.005. Epub 2006 Jul 14.


Lipid profiling is a targeted metabolomics platform that provides a comprehensive analysis of lipid species with high sensitivity. Profiling based on electrospray ionization tandem mass spectrometry (ESI-MS/MS) provides quantitative data and is adaptable to high throughput analyses. Here we report the profiling of 140 apparent molecular species of polar glycerolipids in Arabidopsis leaves, flower stalks, flowers, siliques, roots, and seeds. Considerable differences in lipid species occur among these organs, providing insights into the different lipid metabolic activities in a specific organ. In addition, comparative profiling between wild-type and a knockout mutant pldalpha1 (locus ID: AT3G15730) provides insight into the metabolic function of phospholipase D (PLD) in different organs. PLDalpha1 contributes significantly to phosphatidic acid (PA) levels in roots, seeds, flowers, and flower stalks, but little to basal PA levels in siliques and leaves. In seeds of the pldalpha1 mutant plants, levels of PA, lysophosphatidylcholine, and lysophosphatidylethanolamine were significantly lower than those of wild-type seeds, suggesting a role for PLDalpha1 in membrane lipid degradation in seeds.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Arabidopsis / enzymology
  • Arabidopsis / genetics
  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism
  • Lipids / analysis*
  • Lipids / chemistry
  • Molecular Structure
  • Mutation / genetics*
  • Phosphatidic Acids / analysis
  • Phosphatidic Acids / chemistry
  • Phospholipase D / genetics
  • Phospholipase D / metabolism*
  • Phospholipids / analysis
  • Phospholipids / chemistry
  • Plants, Genetically Modified
  • Principal Component Analysis
  • Spectrometry, Mass, Electrospray Ionization


  • Arabidopsis Proteins
  • Lipids
  • Phosphatidic Acids
  • Phospholipids
  • Phospholipase D