Combined effects of ethanol and manganese on cultured neurons and glia

Neurochem Res. 1991 May;16(5):591-6. doi: 10.1007/BF00974879.


Manganese is essential for normal development and activity of the nervous tissue. Mn2+ ions are involved in protein synthesis and may prevent free radical damage. Since it is now established that alcohol degradation may produce free radicals, we studied the effect of Mn2+ on ethanol induced alterations using cultured nerve cells as an experimental model of the central nervous system. Neurons and glial cells were cultured from rat brain cortex; a tumoral rat glial cell line (C6) was also examined. We measured enzymatic markers of nerve cell maturation (enolase, glutamine synthetase) and superoxide dismutase, a scavenger of free radicals; all these enzymes being activated by Mn2+ ions. Only for the glial cell types an alcohol antagonizing effect was found when Mn2+ was combined with ethanol. Neurons were not sensitive to that Mn2+ effect.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cells, Cultured
  • Ethanol / pharmacology*
  • Free Radicals
  • Glutamate-Ammonia Ligase / metabolism
  • Manganese / pharmacology*
  • Nerve Tissue Proteins / metabolism
  • Neuroglia / drug effects*
  • Neuroglia / metabolism
  • Neurons / drug effects*
  • Neurons / metabolism
  • Phosphopyruvate Hydratase / metabolism
  • Rats
  • Superoxide Dismutase / metabolism


  • Free Radicals
  • Nerve Tissue Proteins
  • Ethanol
  • Manganese
  • Superoxide Dismutase
  • Phosphopyruvate Hydratase
  • Glutamate-Ammonia Ligase