Abstract
To facilitate analysis of plant cell wall polysaccharide structure and composition, we cloned 74 genes encoding polysaccharide-degrading enzymes from Aspergillus nidulans, Aspergillus fumigatus, and Neurospora crassa and expressed the genes as secreted proteins with C-terminal Myc and 6x His tags. Most of the recombinant enzymes were active in enzyme assays, and optima for pH and temperature were established. A subset of the enzymes was used to fragment polysaccharides from the irregular xylem 9 (irx9) mutant of Arabidopsis. The analysis revealed a decrease in the abundance of xylan in the mutant, indicating that the IRX9 gene, which encodes a putative family 43 glycosyltransferase, is required for xylan synthesis.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Arabidopsis / metabolism
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Aspergillus fumigatus / metabolism
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Aspergillus nidulans / metabolism
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Cell Wall / metabolism*
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Gene Expression Regulation, Fungal*
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Genetic Techniques*
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Hydrogen-Ion Concentration
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Molecular Sequence Data
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Neurospora crassa / metabolism
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Plant Proteins / chemistry*
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Plasmids / metabolism
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Polysaccharides / chemistry*
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Temperature
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Xylans / chemistry
Substances
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Plant Proteins
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Polysaccharides
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Xylans
Associated data
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GENBANK/DQ490466
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