Temporal and spatial delineation of mouse Otx2 functions by conditional self-knockout

EMBO Rep. 2006 Aug;7(8):824-30. doi: 10.1038/sj.embor.7400751. Epub 2006 Jul 14.

Abstract

To identify the independent spatial and temporal activities of the essential developmental gene the Otx2, the germline mutation of which is lethal at embryonic day 8.5, we floxed one allele and substituted the other with an inducible CreER recombinase gene. This makes 'trans' self-knockout possible at any developmental stage. The transient action of tamoxifen pulses allows time-course mutation. We demonstrate efficient temporal knockout and demarcate spatio-temporal windows in which Otx2 controls the head, brain structures and body development.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / drug effects
  • Brain / embryology
  • Brain / metabolism
  • Embryo, Mammalian / drug effects
  • Embryo, Mammalian / embryology
  • Embryo, Mammalian / metabolism
  • Embryonic Development / drug effects
  • Embryonic Development / genetics
  • Female
  • Gene Deletion
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental / drug effects
  • Gene Expression Regulation, Developmental / genetics*
  • In Situ Hybridization / methods
  • Male
  • Mice
  • Mice, Inbred Strains
  • Mutagenesis
  • Otx Transcription Factors / genetics*
  • Tamoxifen / administration & dosage
  • Tamoxifen / pharmacology
  • Time Factors

Substances

  • Otx Transcription Factors
  • Otx2 protein, mouse
  • Tamoxifen