Signaling protein SWAP-70 is required for efficient B cell homing to lymphoid organs

Nat Immunol. 2006 Aug;7(8):827-34. doi: 10.1038/ni1365. Epub 2006 Jul 16.

Abstract

The migration of B cells into secondary lymphoid organs is required for the generation of an effective immune response. Here we analyzed the involvement of SWAP-70, a Rac-interacting protein involved in actin rearrangement, in B cell entry into lymph nodes. We noted reduced migration of Swap70-/- B cells into lymph nodes in vivo. Swap70-/- B cells rolled and adhered, yet accumulated in lymph node high endothelial venules. This defect was not due to impaired integrin expression or chemotaxis. Instead, Swap70-/- B cells aberrantly regulated integrin-mediated adhesion. During attachment, Swap70-/- B cells showed defective polarization and did not form uropods or stabilize lamellipodia at a defined region. Thus, SWAP-70 selectively regulates processes essential for B cell entry into lymph nodes.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • B-Lymphocytes / cytology
  • B-Lymphocytes / immunology*
  • Cell Movement / immunology
  • DNA-Binding Proteins / immunology*
  • Guanine Nucleotide Exchange Factors / immunology*
  • Lymph Nodes / cytology
  • Lymph Nodes / immunology*
  • Lymphocyte Activation / immunology
  • Mice
  • Minor Histocompatibility Antigens
  • Nuclear Proteins / immunology*
  • Receptors, Lymphocyte Homing / immunology*

Substances

  • DNA-Binding Proteins
  • Guanine Nucleotide Exchange Factors
  • Minor Histocompatibility Antigens
  • Nuclear Proteins
  • Receptors, Lymphocyte Homing
  • Swap70 protein, mouse