Inhibition of hepatitis B virus by D-fraction from Grifola frondosa: synergistic effect of combination with interferon-alpha in HepG2 2.2.15

Antiviral Res. 2006 Nov;72(2):162-5. doi: 10.1016/j.antiviral.2006.05.011. Epub 2006 Jun 5.


In this study, D-fraction extracted from Grifola frondosa (GF-D) and its combination with human interferon alpha-2b (IFN) were investigated for the inhibitory effect on hepatitis B virus (HBV) in HepG2 2.2.15 cells (2.2.15 cells). HBV DNA and viral antigens were analyzed by a quantitative real-time polymerase chain reaction and end-point titration in radioimmunoassays, respectively. The results showed that GF-D or IFN alone could inhibit HBV DNA in 2.2.15 cells with the 50% inhibitory concentration (IC50) of 0.59 mg/ml and 1399 IU/ml, respectively. We further investigated the combination of GF-D and IFN for anti-HBV activity and found that they synergistically inhibited HBV replication in 2.2.15 cells. In combination with 0.45 mg/ml GF-D, the apparent IC50 value for IFN was 154 IU/ml. This 9-fold increase in antiviral activity of IFN suggested that GF-D could synergize with IFN. These results indicate that GF-D, in combination with IFN, might provide a potentially effective therapy against chronic HBV infections.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antiviral Agents / isolation & purification
  • Antiviral Agents / pharmacology*
  • Cell Line, Tumor
  • DNA, Viral / analysis
  • Drug Synergism
  • Grifola / chemistry*
  • Hepatitis B Antigens / analysis
  • Hepatitis B virus / drug effects*
  • Hepatitis B virus / physiology
  • Humans
  • Interferon alpha-2
  • Interferon-alpha / pharmacology*
  • Polymerase Chain Reaction
  • Recombinant Proteins
  • Virus Replication / drug effects


  • Antiviral Agents
  • DNA, Viral
  • Hepatitis B Antigens
  • Interferon alpha-2
  • Interferon-alpha
  • Recombinant Proteins