Nestin-Cre transgenic mouse line Nes-Cre1 mediates highly efficient Cre/loxP mediated recombination in the nervous system, kidney, and somite-derived tissues

Genesis. 2006 Aug;44(8):355-60. doi: 10.1002/dvg.20226.


Here we describe the generation of the Nes-Cre1 transgenic mouse line in which Cre recombinase expression is controlled by the rat nestin promoter and intron 2 enhancer. This line has previously been used for conditional loss-of-function studies of various genes in the central nervous system and first branchial arch ectoderm. Here we report the detailed temporal and spatial recombination pattern of Nes-Cre1 using three different reporters of Cre-mediated recombination, ROSA26R (R26R), Z/AP, and Z/EG. Cre/loxP recombination was detected in embryos as early as the head-fold stage. By embryonic day (E)15.5 recombination occurred in virtually all cells of the nervous system and unexpectedly also in somite-derived tissues and kidneys. Tissues with little or no recombination included heart, liver, thymus, and lung. This study suggests that Nes-Cre1-mediated recombination occurs in progenitor cell types present in the neuroectoderm, the developing mesonephros, and the somites.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Integrases / genetics
  • Integrases / metabolism*
  • Intermediate Filament Proteins / genetics
  • Kidney / embryology*
  • Mice
  • Mice, Transgenic
  • Nerve Tissue Proteins / genetics
  • Nervous System / embryology*
  • Nestin
  • Recombination, Genetic*
  • Somites / metabolism*
  • Tissue Distribution


  • Intermediate Filament Proteins
  • Nerve Tissue Proteins
  • Nes protein, mouse
  • Nes protein, rat
  • Nestin
  • Cre recombinase
  • Integrases