Cutting edge: unique T cells that recognize citrullinated peptides are a feature of protein immunization

J Immunol. 2006 Aug 1;177(3):1421-5. doi: 10.4049/jimmunol.177.3.1421.

Abstract

Abs against citrullinated proteins are present in patients with rheumatoid arthritis. In this study, we describe a unique cohort of T cells that selectively responded to citrullinated variants of two epitopes of hen egg-white lysozyme, a major and a minor one, bound to the MHC molecule, I-A(k). In addition, we show that when given an intact, unmodified lysozyme protein, dendritic cells and peritoneal macrophages presented citrullinated peptides and stimulated modification-specific T cells. Thus, presentation of citrullinated-peptide-MHC complex is a feature of immune responses to protein Ags.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigen Presentation
  • Antigen-Presenting Cells / immunology
  • Antigen-Presenting Cells / metabolism
  • Cell Line, Tumor
  • Cells, Cultured
  • Citrulline / immunology*
  • Citrulline / metabolism*
  • Epitopes, T-Lymphocyte / immunology
  • Epitopes, T-Lymphocyte / metabolism
  • Freund's Adjuvant / administration & dosage
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Muramidase / administration & dosage
  • Muramidase / immunology
  • Peptide Fragments / administration & dosage
  • Peptide Fragments / immunology*
  • Peptide Fragments / metabolism*
  • T-Lymphocyte Subsets / immunology*
  • T-Lymphocyte Subsets / metabolism*
  • Vaccines, Subunit / administration & dosage*
  • Vaccines, Subunit / immunology*
  • Vaccines, Subunit / metabolism

Substances

  • Epitopes, T-Lymphocyte
  • Peptide Fragments
  • Vaccines, Subunit
  • Citrulline
  • Freund's Adjuvant
  • hen egg lysozyme
  • Muramidase