Direct evidence for enzyme persulfide and disulfide intermediates during 4-thiouridine biosynthesis

Chem Commun (Camb). 2006 Aug 7;(29):3104-6. doi: 10.1039/b604040c. Epub 2006 Jun 14.

Abstract

Two proposed mechanisms for 4-thiouridine generation share key cysteine persulfide and disulfide intermediates, and indirect evidence of their existence has been previously reported; chemical trapping and mass spectrometry have now provided direct and definitive evidence of these key intermediates.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Cysteine / analogs & derivatives*
  • Cysteine / chemistry
  • Disulfides / chemistry*
  • Enzymes / chemistry*
  • Enzymes / metabolism
  • Mass Spectrometry
  • Molecular Structure
  • Thiouridine / chemistry
  • Thiouridine / metabolism*

Substances

  • Disulfides
  • Enzymes
  • Thiouridine
  • cysteine persulfide
  • Cysteine