Development of two multiplex PCR systems for the analysis of 12 X-chromosomal STR loci in a northwestern Italian population sample

C Robino; A Giolitti; S Gino; C Torre

doi:10.1007/s00414-006-0115-9

Development of two multiplex PCR systems for the analysis of 12 X-chromosomal STR loci in a northwestern Italian population sample

Int J Legal Med. 2006 Sep;120(5):315-8. doi: 10.1007/s00414-006-0115-9. Epub 2006 Jul 22.

Authors

C Robino¹, A Giolitti, S Gino, C Torre

Affiliation

¹ Department of Anatomy, Pharmacology and Legal Medicine, University of Turin, c.so Galileo Galilei 22, 10126, Turin, Italy. carlo.robino@unito.it

PMID: 16862443
DOI: 10.1007/s00414-006-0115-9

Abstract

Two multiplex polymerase chain reaction systems for the automated profiling of 12 X-chromosomal short tandem repeat (STR) markers were developed. Multiplex A consisted of DXS6789, DXS6809, GATA172D05, DXS101, DXS8378, and DXS8377. Multiplex B consisted of DXS7132, DXS6800, DXS6801, DXS7424, HPRTB, and DXS10011. The set of amplified X-STRs was designed to include groups of closely linked markers (DXS101-DXS7424 and DXS6789-DXS6801-DXS6809) to generate highly informative haplotypes for kinship testing. A population genetics study of the 12 X-STRs was conducted in a northwestern Italian population sample (n=160, 80 women and 80 men). A diallelic pattern at locus DXS6789 was observed in one man.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromosomes, Human, X / genetics*
Female
Genetics, Population / methods*
Humans
Italy
Male
Microsatellite Repeats
Polymerase Chain Reaction