Abstract
Excitatory amino acid stimulation of phosphatidylinositol (PI) hydrolysis has been associated with development of the CNS. Normally minimally ineffective in stimulating PI hydrolysis in the neonatal rat cerebellum, N-methyl-D-aspartate (NMDA) increased levels of PI hydrolysis 82.3 +/- 5.5% above basal values in the presence of 1 microM baclofen, a gamma-aminobutyric acidB (GABAB) receptor agonist. This effect was observed at day 7 but not in adult cerebellum. The effect of baclofen could be mimicked by low dose GABA and taurine, actions which were blocked by prior application of a specific GABAB antagonist. Therefore, the ability of NMDA to stimulate PI hydrolysis in neonatal cerebellar tissue may be regulated by the degree of GABAB receptor stimulation.
Publication types
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
2-Amino-5-phosphonovalerate / pharmacology
-
6-Cyano-7-nitroquinoxaline-2,3-dione
-
Animals
-
Animals, Newborn
-
Baclofen / pharmacology*
-
Calcium / pharmacology
-
Cerebellum / drug effects
-
Cerebellum / metabolism*
-
N-Methylaspartate / pharmacology*
-
Phosphatidylinositols / metabolism*
-
Quinoxalines / pharmacology
-
Quisqualic Acid / pharmacology*
-
Rats
-
Receptors, GABA-A / drug effects
-
Receptors, GABA-A / physiology*
-
Taurine / pharmacology*
-
gamma-Aminobutyric Acid / analogs & derivatives*
-
gamma-Aminobutyric Acid / pharmacology*
Substances
-
Phosphatidylinositols
-
Quinoxalines
-
Receptors, GABA-A
-
Taurine
-
gamma-Aminobutyric Acid
-
N-Methylaspartate
-
6-Cyano-7-nitroquinoxaline-2,3-dione
-
2-Amino-5-phosphonovalerate
-
Quisqualic Acid
-
Baclofen
-
Calcium