Solar disinfection of poliovirus and Acanthamoeba polyphaga cysts in water - a laboratory study using simulated sunlight

Lett Appl Microbiol. 2006 Aug;43(2):125-30. doi: 10.1111/j.1472-765X.2006.01940.x.


Aims: To determine the efficacy of solar disinfection (SODIS) in disinfecting water contaminated with poliovirus and Acanthamoeba polyphaga cysts.

Methods and results: Organisms were subjected to a simulated global solar irradiance of 850 Wm(-2) in water temperatures between 25 and 55 degrees C. SODIS at 25 degrees C totally inactivated poliovirus after 6-h exposure (reduction of 4.4 log units). No SODIS-induced reduction in A. polyphaga cyst viability was observed for sample temperatures below 45 degrees C. Total cyst inactivation was only observed after 6-h SODIS exposure at 50 degrees C (3.6 log unit reduction) and after 4 h at 55 degrees C (3.3 log unit reduction).

Conclusions: SODIS is an effective means of disinfecting water contaminated with poliovirus and A. polyphaga cysts, provided water temperatures of 50-55 degrees C are attained in the latter case.

Significance and impact of the study: This research presents the first SODIS inactivation curve for poliovirus and provides further evidence that batch SODIS provides effective protection against waterborne protozoan cysts.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acanthamoeba / growth & development
  • Acanthamoeba / isolation & purification
  • Acanthamoeba / radiation effects*
  • Animals
  • Cell Culture Techniques
  • Disinfection / methods*
  • Keratitis / microbiology
  • Poliovirus / growth & development
  • Poliovirus / radiation effects*
  • Sunlight*
  • Virus Inactivation
  • Water / parasitology*
  • Water Microbiology*
  • Water Purification / methods


  • Water