Analysis of cell-based RNAi screens

Genome Biol. 2006;7(7):R66. doi: 10.1186/gb-2006-7-7-R66.


RNA interference (RNAi) screening is a powerful technology for functional characterization of biological pathways. Interpretation of RNAi screens requires computational and statistical analysis techniques. We describe a method that integrates all steps to generate a scored phenotype list from raw data. It is implemented in an open-source Bioconductor/R package, cellHTS ( The method is useful for the analysis and documentation of individual RNAi screens. Moreover, it is a prerequisite for the integration of multiple experiments.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Quality Control
  • RNA Interference*
  • Reproducibility of Results