Isolation and partial sequencing of a pancreatic polypeptide-like neuropeptide from the liver fluke, Fasciola hepatica

R M Magee; C Shaw; I Fairweather; L Thim; C F Johnston; D W Halton

doi:10.1016/0742-8413(91)90031-n

Isolation and partial sequencing of a pancreatic polypeptide-like neuropeptide from the liver fluke, Fasciola hepatica

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1991;100(3):507-11. doi: 10.1016/0742-8413(91)90031-n.

Authors

R M Magee¹, C Shaw, I Fairweather, L Thim, C F Johnston, D W Halton

Affiliation

¹ Comparative Neuroendocrinology Research Group, School of Biology, Queen's University of Belfast, Northern Ireland.

PMID: 1687548
DOI: 10.1016/0742-8413(91)90031-n

Abstract

1. A pancreatic polypeptide (PP)-immunoreactive neuropeptide has been isolated and partially sequenced from the liver fluke, Fasciola hepatica. 2. Gel permeation chromatography of an acid ethanol extract of cattle flukes showed that the peptide is similar in size to mammalian (bovine) PP. 3. The Fasciola peptide was purified to homogeneity by means of reverse-phase HPLC, employing different column chemistries. 4. The purified peptide was sequenced using automated gas-phase Edman degradation and the first 24 amino acid residues determined.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Chromatography, Gel
Chromatography, High Pressure Liquid
Fasciola / metabolism*
Molecular Sequence Data
Pancreatic Polypeptide / chemistry
Pancreatic Polypeptide / isolation & purification*
Radioimmunoassay

Substances

Pancreatic Polypeptide