Catonella morbi and Granulicatella adiacens: new species in endodontic infections

Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006 Aug;102(2):259-64. doi: 10.1016/j.tripleo.2005.09.021. Epub 2006 Apr 21.


Objective: This study intended to examine samples from primary endodontic infections for the presence of Catonella morbi and Granulicatella adiacens, 2 species that have been recently suggested to be involved with infections in other oral sites.

Study design: Genomic DNA was isolated directly from samples taken from teeth with different forms of apical periodontitis, and a devised culture-independent 16S rRNA gene-based heminested PCR assay was used to determine the prevalence of these 2 target species. Species-specific primers were developed by aligning closely related 16S rRNA gene sequences. Species-specificity for each primer pair was confirmed by running PCR against a panel of oral bacteria and by sequencing of DNA from representative positive samples.

Results: C morbi and G adiacens were detected in 33% and 19%, respectively, of the root canals associated with chronic apical periodontitis; 30% and 10%, respectively, of the cases diagnosed as acute apical periodontitis, and 16% and 11%, respectively, of the pus samples taken from acute apical abscesses. Overall, C morbi occurred in 26% and G adiacens in 14% of the samples taken from primary endodontic infections.

Conclusions: Our findings demonstrate that C morbi and G adiacens can take part in the microbiota associated with primary endodontic infections, and their specific role in the disease process warrants further elucidation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • DNA, Bacterial / analysis
  • Gram-Negative Anaerobic Straight, Curved, and Helical Rods / isolation & purification
  • Gram-Negative Anaerobic Straight, Curved, and Helical Rods / pathogenicity*
  • Gram-Negative Bacterial Infections / microbiology
  • Humans
  • Middle Aged
  • Periapical Abscess / microbiology
  • Periapical Periodontitis / microbiology*
  • Polymerase Chain Reaction / methods
  • Ribotyping
  • Sequence Analysis, DNA
  • Streptococcal Infections / microbiology
  • Streptococcus / isolation & purification
  • Streptococcus / pathogenicity*


  • DNA, Bacterial