The Kindlins: subcellular localization and expression during murine development

Exp Cell Res. 2006 Oct 1;312(16):3142-51. doi: 10.1016/j.yexcr.2006.06.030. Epub 2006 Jun 29.


The three Kindlins are a novel family of focal adhesion proteins. The Kindlin-1 (URP1) gene is mutated in Kindler syndrome, the first skin blistering disease affecting actin attachment in basal keratinocytes. Kindlin-2 (Mig-2), the best studied member of this family, binds ILK and Migfilin, which links Kindlin-2 to the actin cytoskeleton. Kindlin-3 is expressed in hematopoietic cells. Here we describe the genomic organization, gene expression and subcellular localization of murine Kindlins-1 to -3. In situ hybridizations showed that Kindlin-1 is preferentially expressed in epithelia, and Kindlin-2 in striated and smooth muscle cells. Kindlins-1 and -2 are both expressed in the epidermis. While both localize to integrin-mediated adhesion sites in cultured keratinocytes Kindlin-2, but not Kindlin-1, colocalizes with E-cadherin to cell-cell contacts in differentiated keratinocytes. Using a Kindlin-3-specific antiserum and an EGFP-tagged Kindlin-3 construct, we could show that Kindlin-3 is present in the F-actin surrounding ring structure of podosomes, which are specialized adhesion structures of hematopoietic cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Adhesion
  • Cell Adhesion Molecules / chemistry
  • Cell Adhesion Molecules / genetics*
  • Cell Adhesion Molecules / metabolism*
  • Cells, Cultured
  • Embryo, Mammalian / cytology
  • Embryo, Mammalian / embryology
  • Embryonic Development / genetics*
  • Fibroblasts / cytology
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental
  • Genome / genetics
  • Hematopoietic System / cytology
  • Humans
  • Mice
  • Mice, Inbred C57BL
  • Myocytes, Smooth Muscle / cytology
  • Protein Transport
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism


  • Cell Adhesion Molecules
  • RNA, Messenger