Characterisation of CTX-M and AmpC Genes in Human Isolates of Escherichia Coli Identified Between 1995 and 2003 in England and Wales

Int J Antimicrob Agents. 2006 Sep;28(3):180-92. doi: 10.1016/j.ijantimicag.2006.03.027. Epub 2006 Aug 1.

Abstract

CTX-M and AmpC genes in human isolates of Escherichia coli, their genetic environment and their host plasmids were examined. Isolates (n=103) were selected based on resistance (minimum inhibitory concentration (MIC)> or =1 microg/mL) to ceftriaxone and cefotaxime. Polymerase chain reaction (PCR) and sequencing identified 29 isolates containing bla(CTX-M-15), 1 each of bla(CTX-M-2) (a strain originating from Israel) and bla(CTX-M-40), 20 isolates containing bla(CMY-7), 4 bla(CMY-2) and 1 bla(CMY-21). This is the first study of plasmid-mediated AmpC genes in E. coli in the UK. Eleven cefoxitin-resistant, AmpC PCR-negative isolates had ampC promoter region mutations. All bla(CTX-M-15) and 24 of 25 bla(CMY) genes were associated with an ISEcp1-like element. The bla(CTX-M-2) was located in an orf513-bearing class 1 integron. Plasmid restriction digests suggest transfer of genes between different plasmid backbones.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Bacterial Proteins / genetics*
  • Cephalosporins / pharmacology
  • Drug Resistance, Bacterial / genetics
  • Electrophoresis, Gel, Pulsed-Field
  • England
  • Escherichia coli / drug effects
  • Escherichia coli / genetics*
  • Escherichia coli / isolation & purification
  • Gene Transfer, Horizontal
  • Humans
  • Microbial Sensitivity Tests
  • Molecular Sequence Data
  • Phylogeny
  • Plasmids
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • Wales
  • beta-Lactamases / genetics*

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Cephalosporins
  • beta-lactamase CTX-2
  • AmpC beta-lactamases
  • beta-Lactamases

Associated data

  • GENBANK/AY607090
  • GENBANK/AY607091
  • GENBANK/AY607092
  • GENBANK/AY607093
  • GENBANK/DQ367336
  • GENBANK/DQ367337
  • GENBANK/DQ367338
  • GENBANK/DQ367339
  • GENBANK/DQ367340
  • GENBANK/DQ367341
  • GENBANK/DQ367342
  • GENBANK/DQ367343