An altered oxidant defense system in red blood cells affects their ability to release nitric oxide-stimulating ATP

Mol Biosyst. 2006 Jun;2(6-7):305-11. doi: 10.1039/b604362n. Epub 2006 May 19.

Abstract

A novel microflow technique is used to demonstrate that a weakened oxidant defense system found in diabetic erythrocytes leads to decreased levels of deformation-induced release of adenosine triphosphate (ATP) from erythrocytes. Addition of an oxidant to rabbit erythrocytes resulted in a 63% decrease in deformation-induced ATP release before eventually recovering to a value that was statistically equivalent to the initial value. Inhibition of glucose-6-phosphate dehydrogenase prevents recovery from the oxidant attack. Finally, results indicated that the ATP release from the erythrocytes of type II diabetics (91 nM +/- 10 nM) was less than half of that measured from the erythrocytes of healthy controls (190 +/- 10 nM). These data suggest that the antioxidant status of erythrocytes is a critical determinant in the ability of these cells to release ATP, a known nitric oxide stimulus.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / metabolism*
  • Adenosine Triphosphate / pharmacology
  • Animals
  • Case-Control Studies
  • Cattle
  • Cell Size
  • Dehydroepiandrosterone / pharmacology
  • Diabetes Mellitus, Type 2 / physiopathology
  • Diamide / pharmacology
  • Dose-Response Relationship, Drug
  • Endothelium, Vascular / metabolism
  • Enzyme Inhibitors / pharmacology
  • Erythrocytes / pathology*
  • Erythrocytes / physiology*
  • Glucosephosphate Dehydrogenase / analysis
  • Glucosephosphate Dehydrogenase / antagonists & inhibitors
  • Glutathione / analysis
  • Glutathione / metabolism
  • Humans
  • Male
  • Models, Biological
  • Nitric Oxide / analysis
  • Nitric Oxide / biosynthesis
  • Nitric Oxide / metabolism*
  • Oxidants / physiology*
  • Oxidation-Reduction
  • Pulmonary Artery / cytology
  • Rabbits
  • Stress, Mechanical
  • Sulfhydryl Reagents / pharmacology
  • Time Factors

Substances

  • Enzyme Inhibitors
  • Oxidants
  • Sulfhydryl Reagents
  • Diamide
  • Nitric Oxide
  • Dehydroepiandrosterone
  • Adenosine Triphosphate
  • Glucosephosphate Dehydrogenase
  • Glutathione