Roles of Two Types of Anion Channels in Glutamate Release From Mouse Astrocytes Under Ischemic or Osmotic Stress

Glia. 2006 Oct;54(5):343-57. doi: 10.1002/glia.20400.

Abstract

Astrocytes release glutamate upon hyperexcitation in the normal brain, and in response to pathologic insults such as ischemia and trauma. In our experiments, both hypotonic and ischemic stimuli caused the release of glutamate from cultured mouse astrocytes, which occurred with little or no contribution of gap junction hemichannels, vesicle-mediated exocytosis, or reversed operation of the Na-dependent glutamate transporter. Cell swelling and chemical ischemia activated, in cell-attached membrane patches, anionic channels with large unitary conductance (approximately 400 pS) and inactivation kinetics at potentials more positive than +20 mV or more negative than -20 mV. These properties are different from those of volume-sensitive outwardly rectifying (VSOR) Cl- channels, which were also expressed in these cells and exhibited intermediate unitary conductance (approximately 80 pS) and inactivation kinetics at large positive potentials of more than +40 mV. Both maxi-anion channels and VSOR Cl- channels were permeable to glutamate with permeability ratios of glutamate to chloride of 0.21 +/- 0.07 and 0.15 +/- 0.01, respectively. However, the release of glutamate was significantly more sensitive to Gd3+, a blocker of maxi-anion channels, than to phloretin, a blocker of VSOR Cl- channels. We conclude that these two channels jointly represent a major conductive pathway for the release of glutamate from swollen and ischemia-challenged astrocytes, with the contribution of maxi-anion channels being predominant.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn
  • Astrocytes / drug effects
  • Astrocytes / metabolism*
  • Brain / cytology
  • Brain / metabolism*
  • Brain / physiopathology
  • Brain Edema / metabolism*
  • Brain Edema / physiopathology
  • Brain Ischemia / metabolism
  • Brain Ischemia / physiopathology
  • Cell Membrane / drug effects
  • Cell Membrane / metabolism
  • Cell Membrane Permeability / drug effects
  • Cell Membrane Permeability / physiology
  • Cells, Cultured
  • Chloride Channels / drug effects
  • Chloride Channels / metabolism
  • Chlorides / metabolism
  • Chlorides / pharmacology
  • Gadolinium / pharmacology
  • Glutamic Acid / metabolism*
  • Glutamic Acid / pharmacology
  • Hypotonic Solutions / pharmacology
  • Membrane Potentials / drug effects
  • Membrane Potentials / physiology
  • Mice
  • Osmotic Pressure / drug effects
  • Patch-Clamp Techniques
  • Stress, Physiological / metabolism
  • Stress, Physiological / physiopathology
  • Voltage-Dependent Anion Channels / drug effects
  • Voltage-Dependent Anion Channels / metabolism*

Substances

  • Chloride Channels
  • Chlorides
  • Hypotonic Solutions
  • Voltage-Dependent Anion Channels
  • Glutamic Acid
  • Gadolinium