Methodological approach to minimal residual disease detection by flow cytometry in adult B-lineage acute lymphoblastic leukemia

Haematologica. 2006 Aug;91(8):1109-12.


A flow cytometric approach to minimal residual disease (MRD) monitoring useful in childhood B-lineage acute lymphoblastic leukemia (ALL) is discussed here in the context of ALL in adults. Of 64 leukemia samples analyzed, 95.3% had at least one abnormal phenotype (57.3% had two or more) as compared to physiologic B-cell precursors in adult bone marrow. The method was sensitive enough to detect one leukemic cell among 10,000 normal cells in 16/19 experiments (84.2%). Blast phenotypes were stable in culture and at relapse, and were useful for MRD monitoring in patients. Marker combinations for childhood ALL are also applicable to adult cases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antibodies, Monoclonal
  • Antigens, CD / analysis
  • B-Lymphocytes / cytology
  • B-Lymphocytes / immunology
  • B-Lymphocytes / pathology
  • Bone Marrow Cells / cytology
  • Bone Marrow Cells / pathology
  • Burkitt Lymphoma / pathology*
  • Child
  • Flow Cytometry / methods*
  • Fluorescein-5-isothiocyanate
  • Humans
  • Neoplasm, Residual / pathology*
  • Reference Values
  • Regeneration


  • Antibodies, Monoclonal
  • Antigens, CD
  • Fluorescein-5-isothiocyanate