Abstract
Resveratrol production in Saccharomyces cerevisiae was compared to that in Escherichia coli. In both systems, 4-coumarate:coenzyme A ligase from tobacco and stilbene synthase from grapes were expressed. When p-coumaric acid was used as the precursor, resveratrol accumulations in the culture medium were observed to be comparable in E. coli (16 mg/liter) and yeast (6 mg/liter).
MeSH terms
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Acyltransferases / genetics
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Acyltransferases / metabolism
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Biotechnology / methods
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Coenzyme A Ligases / genetics
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Coenzyme A Ligases / metabolism
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Coumaric Acids / metabolism
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Escherichia coli / enzymology*
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Escherichia coli / genetics*
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Escherichia coli / metabolism
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Nicotiana / enzymology
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Nicotiana / genetics
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Recombination, Genetic*
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Resveratrol
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Saccharomyces cerevisiae / enzymology*
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae / metabolism
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Stilbenes / metabolism*
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Vitis / genetics
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Vitis / metabolism
Substances
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Coumaric Acids
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Stilbenes
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Acyltransferases
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stilbene synthase
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Coenzyme A Ligases
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4-coumarate-CoA ligase
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Resveratrol