Prostaglandin E2 receptor subtype 2 (EP2) null mice are protected against murine lung tumorigenesis

Anticancer Res. 2006 Jul-Aug;26(4B):2857-61.

Abstract

Background: Manipulating prostaglandin (PG) production modulates tumor development. Elevated PGI2 production prevents murine lung cancer, while decreasing PGE2 content protects against colon cancer. PGE2 receptor subtype 2 (EP2) -deficient mice were hypothesized to be resistant to lung tumorigenesis.

Materials and methods: EP2 null BALB/c mice and their wild-type littermates were exposed to an initiation-promotion carcinogenesis protocol and lung tumorigenesis was examined. Chronic lung inflammation was induced to determine whether EP2 ablation influenced inflammatory cell infiltration.

Results: Tumor multiplicity in EP2 null mice was 34% lower than in their wild-type littermates (21.9+/-3.0 vs. 14.5+/-2.9 tumors/mouse, p<0.001). The lung tumor burden, an indicator of growth rate, also declined (57%, p<0.05). All the mice exhibited similar inflammatory cell infiltration.

Conclusion: PGE2, acting through EP2, enhanced lung tumorigenesis through a mechanism that may be distinct from its proinflammatory activity. Thus, EP2 is a potential target for novel chemoprevention strategies.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Butylated Hydroxytoluene
  • Cell Growth Processes / physiology
  • Female
  • Leukocytes / immunology
  • Lung Neoplasms / chemically induced
  • Lung Neoplasms / metabolism
  • Lung Neoplasms / pathology
  • Lung Neoplasms / prevention & control*
  • Male
  • Methylcholanthrene
  • Mice
  • Mice, Inbred BALB C
  • Mice, Knockout
  • Receptors, Prostaglandin E / deficiency*
  • Receptors, Prostaglandin E / genetics
  • Receptors, Prostaglandin E, EP2 Subtype

Substances

  • Ptger2 protein, mouse
  • Receptors, Prostaglandin E
  • Receptors, Prostaglandin E, EP2 Subtype
  • Butylated Hydroxytoluene
  • Methylcholanthrene