Detection of platelet activation with monoclonal antibodies and flow cytometry. Changes during platelet storage

Transfusion. 1990 Jan;30(1):20-5. doi: 10.1046/j.1537-2995.1990.30190117623.x.


To investigate whether changes in platelet condition during platelet storage correlate with an altered expression of platelet membrane proteins, the binding of monoclonal antibodies (MoAbs) to fresh platelets was compared with MoAbs' binding to thrombin-activated platelets and to platelets stored as platelet concentrates. The MoAbs included antibodies against the platelet glycoprotein (GP) IIb/IIIa complex and against two activation-dependent antigens, one of which was a component of the internal platelet alpha-granule membrane (GMP 140) and the other of which was a 53-kD protein derived from platelet lysosomes. The binding of MoAbs to platelets fixed with 1 percent paraformaldehyde was measured by flow cytometry. In thrombin-activated platelets, a threefold increase was found in the expression of GP IIb/IIIa over that in fresh platelets. The binding of the activation-dependent MoAbs increased from 2 to 3 percent to 70 to 80 percent of the platelets. Storage of platelet concentrates for 5 days resulted in a 60 percent increase in GP IIb/IIIa expression compared to Day 0 and increased binding of the MoAbs directed against GMP-140 from 3 to 16 percent and against the 53-kD protein from 2 to 8 percent of the platelets, respectively. These changes correlated with modifications in platelet morphology (decrease in swirling), leakage of lactate dehydrogenase, and release of beta-thromboglobulin. These data indicate that platelets become activated and are damaged during the storage of platelet concentrates.(ABSTRACT TRUNCATED AT 250 WORDS)

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal*
  • Blood Platelets / immunology
  • Blood Platelets / physiology*
  • Blood Platelets / ultrastructure
  • Blood Pressure*
  • Flow Cytometry*
  • Humans
  • L-Lactate Dehydrogenase / blood
  • Lysosomes / immunology
  • P-Selectin
  • Platelet Activation* / drug effects
  • Platelet Membrane Glycoproteins / immunology
  • Platelet Membrane Glycoproteins / metabolism
  • Thrombin / pharmacology
  • beta-Thromboglobulin / metabolism


  • Antibodies, Monoclonal
  • P-Selectin
  • Platelet Membrane Glycoproteins
  • beta-Thromboglobulin
  • L-Lactate Dehydrogenase
  • Thrombin