NAP, a peptide derived from the activity-dependent neuroprotective protein, modulates macrophage function

Ann N Y Acad Sci. 2006 Jul;1070:500-6. doi: 10.1196/annals.1317.069.


NAP is an eight-amino acid neuroprotective peptide NAPVSIPQ; it is the smallest active element derived from the recently cloned activity-dependent neuroprotective protein (ADNP). NAP readily enters the brain from the blood. It will be important to learn whether NAP, in addition to its neuroprotective activity, also might influence immune-mediated inflammation. Here, we report that: (a) macrophages express ADNP; (b) expression of ADNP in macrophages responds to VIP; and (c) NAP downregulates the key inflammatory cytokines tumor necrosis factor (TNF-alpha), interleukin-16 (IL-16), and IL-12 in macrophages. These findings indicate that ADNP/NAP can play an important role in immune regulation as well as in neuroprotection, which may be mutually related processes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cell Line
  • Cytokines / metabolism
  • Gene Expression Regulation
  • Homeodomain Proteins / genetics
  • Macrophages / drug effects*
  • Macrophages / metabolism*
  • Mice
  • Nerve Tissue Proteins / genetics
  • Neuroprotective Agents / chemistry
  • Neuroprotective Agents / pharmacology*
  • Oligopeptides / chemistry
  • Oligopeptides / pharmacology*


  • Adnp protein, mouse
  • Cytokines
  • Homeodomain Proteins
  • Nerve Tissue Proteins
  • Neuroprotective Agents
  • Oligopeptides
  • davunetide