Determination and validation of ketoprofen, pantoprazole and valsartan together in human plasma by high performance liquid chromatography

Pharmazie. 2006 Jul;61(7):586-9.


A rapid and specific high-performance liquid chromatographic method was developed and validated for the simultaneous determination of ketoprofen, valsartan and pantoprazole in human plasma. Chromatographic separation of ketoprofen, valsartan and pantoprazole was performed using a Chromasil C18 column (250 mm x 4.6 mm i.d., 5 microm particle size). The mobile phase consisted of a mixture of 0.02 M sodium dihydrogen phosphate buffer (pH 3.15) and acetonitrile (58:42, v/v) pumped through the chromatographic system at a flow rate of 1 mL x min(-1). The Diode Array detector was operated at 225 and 272 nm. Rofecoxib was used as an internal standard. Sample treatment procedure consisted of deproteinisation with acetonitrile-methanol (50:50 v/v). Analytical recoveries were in the range of 79.00-118.00% of nominal values of valsartan, ketoprofen and pantoprazole. The method was reproducible and accurate with lower limits of quantification 250 microg x L(-1) for pantoprazole and 500 microg x L(-1) for ketoprofen and valsartan. This method was relatively easy to perform and allows simultaneous determination of these three drugs in plasma at nanogram levels.

MeSH terms

  • 2-Pyridinylmethylsulfinylbenzimidazoles
  • Anti-Inflammatory Agents, Non-Steroidal / blood*
  • Anti-Ulcer Agents / blood*
  • Antihypertensive Agents / blood*
  • Benzimidazoles / blood*
  • Chromatography, High Pressure Liquid
  • Humans
  • Indicators and Reagents
  • Ketoprofen / blood*
  • Omeprazole / analogs & derivatives*
  • Omeprazole / blood
  • Pantoprazole
  • Reference Standards
  • Reproducibility of Results
  • Sulfoxides / blood*
  • Tetrazoles / blood*
  • Valine / analogs & derivatives*
  • Valine / blood
  • Valsartan


  • 2-Pyridinylmethylsulfinylbenzimidazoles
  • Anti-Inflammatory Agents, Non-Steroidal
  • Anti-Ulcer Agents
  • Antihypertensive Agents
  • Benzimidazoles
  • Indicators and Reagents
  • Sulfoxides
  • Tetrazoles
  • Valsartan
  • Ketoprofen
  • Pantoprazole
  • Valine
  • Omeprazole